FIG. 5.

The oriT region of the pMV158 family of plasmids. (A) Features of the pMV158 oriT and its conservation among RCR-plasmids. The inverted repeats (IR) are indicated by arrows above and below the sequence. Nucleotides protected from DNase I cleavage by MobM are indicated in boldface italics, whereas the extended −10 promoter region of the mobM gene is underlined. The last nucleotide depicted (G, underlined) is the mobM transcription initiation site in L. lactis. The MobM-mediated nick (l) and the changes in the nucleotide sequence (boldface) are indicated. (B) Characterized oriT regions of three plasmids with similar mobilization cassette and the same nick site (l). Differences are underlined. DNA from plasmid pVA380-1 is a substrate of pMV158 MobM.