FIG. 1.

Alignment of ADP-Glc PPases from different classes. Amino acid alignment was performed with the program PILEUP from the Wisconsin package (http://www.gcg.com). The alignment was fine tuned manually based on the secondary structure of each enzyme as predicted by the PHD program (81). Residues in blue and red were predicted to be β-sheets and α-helixes, respectively; pale shades indicate a lower level of confidence. Green residues were predicted to be neither of these (loops). In black are residues for which the PHD program could not make a prediction. Insertions and deletions were introduced to maximize the alignment of both primary and secondary structure. a, sequence of the small (catalytic) subunit; b, sequence of the subunit encoded by glgC (catalytic).