TABLE 1.

Percent inhibition of MAbs by blocking ELISA using sera from WNV-infected horses^c

Serum sourcea	Day post- challenge	WNV PRNT90 titer	% Inhibition of binding of MAbb:
			2B2	3.1112G	3H6	6B6C-1
H1	0	<10	0	4.5	3.8	4.1
H2	0	<10	3.4	2.7	3.4	5.0
H3	0	<10	0	1.3	1.7	3.8
H4	0	<10	0	2.5	4.7	5.6
H5	0	<10	5.2	0.6	2.6	2.6
H6	0	<10	0	2.9	4.0	4.2
H7	0	<10	11.7	11.0	1.2	0
H8	0	<10	1.4	0	3.0	0
H9	0	<10	3.3	5.2	0.3	0
Mean			2.8	3.4	2.7	2.8
H1	7	<10	0	0	0.8	0.2
H2	7	<10	4.9	0	0	0
H3	7	<10	4.2	1.6	0	16.2
H4	7	<10	6.4	5.6	10.3	13.9
H5	7	<10	10.9	0	0	18.6
H6	7	<10	10.0	7.4	1.3	4.1
H7	7	<10	0	0.8	0	0
H8	7	<10	13.0	6.6	0	0
H9	7	<10	0	3.0	3.3	0
Mean			5.5	2.8	1.7	5.9
H1	14	160	45.3	34.3	34.8	34.6
H2	14	80	31.0	34.1	20.9	39.9
H3	14	160	24.1	55.6	34.5	56.9
H4	14	80	18.0	64.3	35.0	57.5
H5	14	160	57.0	57.6	39.6	65.8
H6	14	160	36.6	58.7	35.7	58.1
H7	14	80	8.7	46.0	27.1	56.0
H8	14	≥160	30.6	69.0	29.6	49.8
H9	14	≥160	45.0	80.5	47.8	59.1
Mean			32.9	55.6	33.9	53.1
H1	21	160	58.0	69.7	51.5	57.9
H2	21	40	36.0	60.7	38.7	57.5
H3	21	40	34.4	64.7	50.2	61.9
H4	21	40	41.1	75.2	52.7	72.3
H5	21	40	76.7	69.3	43.9	68.0
Mean			49.2	67.9	47.4	63.5
H1	28	≥160	48.5	74.0	54.5	65.6
H2	28	≥160	60.5	72.8	53.4	73.6
H4	28	80	40.0	79.5	54.2	70.8
Mean			49.7	75.4	54.0	70.0
H10d	31	320	77.2	28.8	44.3	66.2

^aAll sera were diluted 10-fold and tested in duplicate. Control serum consisted of commercially available normal horse serum (Vector Laboratories, Burlingame, Calif.) or a pooled suspension of sera collected from H1 to H9 at day 0.

^bMAb 2B2 is WNV and KOUV specific; MAb 3.1112G is WNV specific; MAbs 3H6 and 6B6C-1 are flavivirus group reactive.

^cBoldface inhibition values (≥30%) and boldface PRNT titers (≥20) were considered significant.

^dHorse was vaccinated prior to virus challenge.