TABLE 1.
Interpretation of WB pattern
| Conclusion | Patterna
|
No. of subjectsb
|
||||||
|---|---|---|---|---|---|---|---|---|
| GD21 | P19 | P24 | rgp-46I | rgp-46II | WB | PCR (tax) | PCR (LTR) | |
| HTLV-1 | + | + | +/− | + | − | 208 | 50+ | 50+ |
| HTLV-2 | + | +/− | + | − | + | 0 | 0 | 0 |
| HTLVbc | + | + | + | − | − | 11 | 11 (HTLV-1+) | 11 (HTLV-1+) |
| Indeterminant | + | − | + | − | − | 3 | Negative | Negative |
| Indeterminant | + | + | − | − | − | 5 | Negative | Negative |
a
rgp-46I/II, type-specific recombinant Env proteins; P19 and P24, Gag proteins; GD21, Env protein.
b
For PCR (tax) and PCR (LTR), of 208 positive samples, 50 were randomly tested for tax and LTR regions of the HTLV-1 genome by PCR amplification, which confirmed the WB results.
c
All HTLV WB-positive specimens were determined to be HTLV-1 positive by PCR amplification.