TABLE 3.
In vitro activities (MICs) of six antimicrobial agents for control strains and for reference and type strains of Brachyspira spp. tested in the broth dilution panela
| Strain | Medium | MIC (μg/ml) of:
|
|||||
|---|---|---|---|---|---|---|---|
| Tiamulin | Tylosin | Erythromycin | Clindamycin | Virginiamycin | Carbadox | ||
| Bacteroides fragilis (ATCC 25285) | BHIS | 1-4 (9) | ≤2 (9) | 2-4 (9) | 0.25b-1 (9) [0.5-2c] | 4-8 (9) | ≥0.5 (7) |
| Eubacterium lentum (ATCC 43055) | BHIS | ≥2 (9) | ≤2 (9) | ≤2 (9) | 0.125-0.25d (9) [0.03-0.12c] | 1-4 (9) | ≥0.5 (7) |
| Escherichia coli (ATCC 25922) | MH | >2 (9) | >256 (9) [>64e] | 64-128 (9) | >4 (9) | >16 (9) | >0.5 (5) |
| Staphylococcus aureus (ATCC 29213) | MH | 1 (12) [0.5-2f] | ≤2 (12) [1-2e] | ≤2 (12) [0.25-1g] | 0.125-0.25 (12) [0.06-0.25g] | 1-2 (12) | >0.5 (5) |
| B. hyodysenteriae B78T (ATCC 27164T) | BHIS | 0.03-0.06 (7) | 4-8 (7) | 4-32 (6) | 0.06-0.125 (7) | 1-2 (7) | 0.016-0.06 (7) |
| B. hyodysenteriae B204 (ATCC 31212) | BHIS | 0.03-0.06 (6) | 64->256 (6) | >256 (6) | 1->4 (6) | 1-2 (6) | 0.016-0.03 (5) |
| B. hyodysenteriae 84193-2x/99 (CCUG 47386) | BHIS | 8-16 (5) | >128 (2) | NT | NT | NT | NT |
| B. intermedia PWS/AT (ATCC 51140T) | BHIS | 0.03-0.06 (3) | 4-8 (3) | 2-16 (3) | 0.03-0.06 (3) | 1 (3) | 0.008-0.03 (3) |
| B. murdochii 56-150T (ATCC 51284T) | BHIS | 0.06-0.125 (3) | >256 (3) | >256 (3) | ≥4 (3) | 1-2 (3) | 0.03-0.06 (3) |
a
Numbers in parentheses represent numbers of tests performed. Numbers in brackets represent MIC ranges. MH, Mueller-Hinton broth. NT, not tested.
b
Eight of nine tests were within the range.
c
Approved by the NCCLS (18).
d
Five of nine tests were within the range.
e
Proposed by Odland et al. (21).
f
Approved by the NCCLS (19).
g
Approved by the NCCLS (20).