TABLE 2.
Nucleotide and amino acid differences between blaEcloMHN1, blaEcloK992004.1, blaEcloK995120.1, blaEcloK99230, blaEareK9911729, blaEcloGC1, blaEcloCHE, blaEcloQ908R, blaEcloK9973, blaEcloK9914325, and blaEcloP99
| Nucleotide no. | Amino acid no.a | Nucleotide (amino acid)b encoded by gene (accession no. or reference):
|
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| blaEcloMHN1 (X08082) | blaEcloK992004.1 (this study) | blaEcloK995120.1 (this study) | blaEcloK99230 (this study) | blaEareK9911729 (this study) | blaEcl oGC1 (D44479) | blaEcloCHE (AJ278994) | blaEcloQ908R (X08081) | blaEcloK9973 (this study) | blaEcloK9914325 (this study) | blaEcloP99 (X07274) | ||
| 21 | 3 | T (Ile) | T | T | T | T | A (Lys) | A (Lys) | ND | G (Arg) | G (Arg) | G (Arg) |
| 49 | 12 | G (Leu) | G | G | G | G | C | C | C | C | C | C |
| 53 | 14 | C (Leu) | C | C | C | C | A (Ile) | A (Ile) | A (Ile) | A (Ile) | A (Ile) | A (Ile) |
| 55 | 14 | C (Leu) | C | C | C | C | C (Ile) | T (Ile) | C (Ile) | C (Ile) | C (Ile) | C (Ile) |
| 74 | 21 | G (Ala) | G | G | G | G | A (Thr) | A (Thr) | A (Thr) | A (Thr) | A (Thr) | A (Thr) |
| 82 | 23 | A (Val) | A | G | A | A | G | G | G | G | G | G |
| 109 | 32 | C (Val) | C | C | C | C | A | C | C | C | C | C |
| 119 | 36 | G (Val) | G | G | G | G | G | G | G | G | G | A (Ile) |
| 185 | 58 | T (Ser) | T | T | C (Pro) | C (Pro) | C (Pro) | C (Pro) | C (Pro) | C (Pro) | C (Pro) | C (Pro) |
| 199 | 62 | G (Thr) | G | G | G | G | G | G | G | G | G | A |
| 262 | 83 | T (Gly) | T | C | T | T | T | T | T | T | T | T |
| 289 | 92 | T (Val) | T | T | T | T | G | T | T | T | T | T |
| 335 | 108 | C (Pro) | C | C | C | C | C | C | C | C | C | G (Ala) |
| 373 | 120 | G (Gln) | G | G | G | G | G | A | G | G | G | G |
| 466 | 151 | G (Leu) | G | G | G | G | G | G | C | G | G | G |
| 467 | 152 | C (Leu) | C | C | C | C | C | C | G (Val) | C | C | C |
| 571 | 186 | T (Ser) | T | T | C | C | T | T | T | T | T | T |
| 610 | 199 | T (Leu) | T | G | T | T | T | T | T | T | T | T |
| 646 | 211 | T (Val) | T | T | T | T | G | G | G | G | G | G |
| 697 | 228 | G (Ala) | G | G | G | G | I-9 | G | G | G | G | G |
| 715 | 234 | A (Gly) | A | A | A | A | T | T | T | T | T | T |
| 717 | 235 | T (Met) | T | T | C (Thr) | C (Thr) | T | T | T | T | T | T |
| 790 | 259 | G (Ala) | G | G | G | G | A | G | G | G | G | G |
| 796 | 261 | G (Glu) | G | G | A | A | G | G | G | G | G | G |
| 799 | 262 | G (Lys) | G | G | G | G | C (Asn) | C (Asn) | C (Asn) | C (Asn) | C (Asn) | C (Asn) |
| 805 | 264 | C (Ala) | C | C | C | C | T | T | T | T | T | T |
| 820 | 269 | G (Lys) | G | G | G | G | A | G | G | G | G | G |
| 928 | 305 | A (Glu) | A | A | A | A | G | G | G | G | G | G |
| 937 | 308 | C (Asp) | C | C | C | C | C | D-18 | C | C | C | C |
| 949 | 312 | G (Ala) | G | G | G | G | G | G | G | G | G | A |
| 961 | 316 | A (Leu) | A | A | A | A | G | G | G | G | G | G |
| 967 | 318 | G (Val) | A | G | G | G | G | G | G | G | G | G |
| 969 | 319 | C (Ala) | C | C | C | C | C | T (Val) | T (Val) | T (Val) | T (Val) | C |
| 1024 | 337 | T (Gly) | T | T | T | T | C | C | C | C | C | C |
| 1030 | 339 | C (Thr) | C | T | T | T | T | T | T | T | T | T |
| 1098 | 362 | A (Lys) | A | A | A | A | C (Thr) | C (Thr) | C (Thr) | C (Thr) | C (Thr) | C (Thr) |
a
Numbering is according to that of blaEcloMHN1 (X08082).
b
The encoded amino acid was indicated when there was a point mutation leading to an amino acid change relative to the EcloMHN1 sequence. Abbreviations: ND, not determined; I-9, 9 nucleotides (GGTGCGCGC) inserted between the 696th and 697th nucleotides; D-18, 18 nucleotides (AGTAAGGTAGCGCTGGCG) deleted between the 937th and 956th nucleotides. Abbreviations of gene names are given in Materials and Methods.