TABLE 5.
Comparison of formulations for BM and BD standard aerobic mediaa
| Characteristic | Formulation for:
|
|
|---|---|---|
| BM medium | BD medium | |
| Vol (ml) | 40 | 40 |
| Pancreatic digest of casein (% [wt/vol]) | 1.7 | |
| Pancreatic digest of soybean meal (% [wt/vol]) | 0.3 | |
| Soybean-casein digest broth (% [wt/vol]) | 3.0 | |
| Sodium polyanethole sulfonate (% [wt/vol]) | 0.035 | 0.025 |
| Pyridoxine or pyridoxal HCl (% [wt/vol]) | 0.001 | 0.001 |
| Other complex amino acids and carbohydrate substrates in purified water | As needed | |
| Atmosphere | CO2 in oxygen under vacuum | CO2 |
| Other proprietary adjustments | As needed | |
| Yeast extract (% [wt/vol]) | 0.3 | |
| Animal tissue digest (% [wt/vol]) | 0.01 | |
| Sucrose (% [wt/vol]) | 0.1 | |
| Hemin (% [wt/vol]) | 0.0005 | |
| Menadione (% [wt/vol]) | 0.00005 | |
| Sodium bicarbonate (% [wt/vol]) | 0.04 | |
a
Medium formulations derived from respective manufacturers’ package inserts.