TABLE 2.
Oligonucleotides for amplification and sequencing of the lag-1 region
| Oligo- nucleotide | Sequence (5′-3′) | Source or reference |
|---|---|---|
| lag-Corby 34u | GTCTTGGCCGGACATACTTTATCAG | 21 |
| lag-Corby Ku | CTGAAAGTGGACTGATTACAAGAGAG | 21 |
| lag-Corby Pd | GCTTGCGGTTTTCCCATTTTATCC | 21 |
| lag-Corby 540u | GTTCTGGGTGCGCATATCATTTTTG | 21 |
| lag-Corby 564d | CAAAAATGATATGCGCACCCAGAAC | 21 |
| lag-Corby 58d | CTGATAAAGTATGTCCGGCCAAGAC | 21 |
| lag-Corby Ed | CTATATTGTGAGGACTACGATTGG | This study |
| lag-Phil Bd | CGTCGGCTCAATAAATGTGGATAC | This study |
| lag-Phil Bu | GTATCCACATTTATTGAGCCGACG | This study |
| lag-Phil Dd | GGCTATCCCAAACAAAGTATACAAC | This study |
| lag-Phil Du | GTTGTATACTTTGTTTGGGATAGCC | This study |
| lag-Phil Gu | GATGCCAAATCCGCTTTCACTAAC | This study |
| lag-Phil Jd | CCTGATAAAGTATGACCAGCCAAGAC | This study |
| lag-Phil Ju | GTCTTGGCTGGTCATACTTTATCAGG | This study |
| lag-Phil Ld | CTCACCCCATTTATACTCTATCTTAAC | This study |
| lag-Phil Lu | GTTAAGATAGAGTATAAATGGGGTGAG | This study |
| ORF-1 dorc | CTAAAATTGATGCGATTAAAGGTTTG | This study |
| ORF-2 do | GAGTGGTAGCTGGAGAAGAATTTTC | This study |
| ORF-2 dorc | GAAAATTCTTCTCCAGCTACCACTC | This study |
| ORF-2 up | CATACAATGCACCAACACAATGATG | This study |
| ORF-2 upre | CATCATTGTGTTGGTGCATTGTATG | This study |
| ORF-3 do | TCGACGATGAGGAAACAGAAATAAT | This study |
| ORF-3 dore | ATTATTTCTGTTTCCTCATCGTCGA | This study |
| ORF-3 up | GCCGATCATACCGCACAAGTA | This study |
| ORF-3 upre | TACTTGTGCGGTATGATCGGC | This study |
| ORF-4 do | CCCCCTCATTTACAGCTCCATC | This study |
| ORF-4 dore | GATGGAGCTGTAAATGAGGGGG | This study |
| ORF-4 up | GGGGGCGCTTAATCAGTTTG | This study |