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Standard curves of four dengue virus serotypes tested by one-step SYBR Green-based quantitative RT-PCR using serotype-specific primer pair. Standard curves were generated from the amplification plots of each of the four dengue strains representing DEN-1 (A), DEN-2 (B), DEN-3 (C), and DEN-4 (D). The starting viral titer was plotted against the Ct value of each dilution.
