TABLE 3.
Comparison of results obtained by one-step SYBR Green-based RT-PCR assay and cell culture method for detection of dengue virus in acute-phase seraa
| Serotype | No. of confirmed casesb | No. (%) of positive serum samples detected by:
|
||
|---|---|---|---|---|
| Virus isolation | Group-specific primer pair | Serotype-specific primer pairs | ||
| DEN-1 | 8 | 5 (63) | 6 (75) | 6 (75) |
| DEN-2 | 162 | 108 (67) | 132 (82) | 132 (82) |
| DEN-3 | 13 | 10 (77) | 12 (92) | 12 (92) |
| DEN-4 | 10 | 6 (60) | 10 (100) | 10 (100) |
| Total | 193 | 129 (67) | 160 (83) | 160 (83) |
a
Sera collected during days 1 to 7 after onset of symptoms are referred to as acute-phase samples.
b
All serum samples were selected from patients with confirmed cases of dengue for whose samples dengue virus isolation in the acute phase was positive or for whom a ≥4-fold increase of dengue-specific IgM or IgG antibodies in the serum pair was demonstrated.