TABLE 2.
PCR primers used in this study
| Designation | Sequence (5′ to 3′) | Target gene | Amplicon size (bp) | Reference |
|---|---|---|---|---|
| SK1 | CCCGAATTCGGCACAAGCATAAGC | eae | 881 | 10 |
| SK2 | CCCGGATCCGTCTCGCCAGTATTCG | |||
| VTcom-u | GAGCGAAATAATTTATATGTG | stx | 518 | 21 |
| VTcom-d | TGATGATGGCAATTCAGTAT | |||
| AL65 | TTAATAGCACCCGGTACAAGCAGG | est | 147 | 4 |
| AL125 | CCTGACTCTTCAAAAGAGAAAATTAC | |||
| LTL | TCTCTATGTGCATACGGAGC | elt | 322 | 20 |
| LTR | CCATACTGATTGCCGCAAT | |||
| ipaIII | GTTCCTTGACCGCCTTTCCGATACCGTC | ipaH | 619 | 17 |
| ipaIV | GCCGGTCAGCCACCCTCTGAGAGTAC | |||
| aggRks1 | GTATACACAAAAGAAGGAAGC | aggR | 254 | 14 |
| aggRkas2 | ACAGAATCGTCAGCATCAGC | |||
| Eaggfp | AGACTCTGGCGAAAGACTGTATC | CVD432 | 194 | 11 |
| Eaggbp | ATGGCTGTCTGTAATAGATGAGAAC | |||
| aspU-3 | GCCTTTGCGGGTGGTAGCGG | aspU | 282 | This study |
| aspU-2 | AACCCATTCGGTTAGAGCAC |