Figure 5.

Effect of pretreatment with an antisense oligonucleotide (aODN) to mKv1.1 gene on clonidine- and guanabenz-induced antinociception in the mouse hot-plate test. (a) Prevention by aODN (2.0 nmol per single i.c.v. injection) of clonidine (0.05–0.20 mg kg⁻¹, s.c.) antinociception. The test was performed 72 h after the last i.c.v. injection of degenerate ODN (dODN; 2.0 nmol per single i.c.v. injection) or aODN. Vertical lines represent s.e.mean; between 12 and 16 mice were tested. ^*P<0.05 in comparison with dODN+clonidine group. (b) Prevention by aODN (2.0 nmol per single i.c.v. injection) of guanabenz (0.02–0.50 mg kg⁻¹, s.c.) antinociception. The test was 72 h after the last i.c.v. injection of degenerate ODN (dODN; 2.0 nmol per single i.c.v. injection) or aODN. Vertical lines represent s.e.mean; between 13 and 19 mice were tested. ^*P<0.05; ^**P<0.01 in comparison with dODN+guanabenz group.