Figure 2.

Concentration-response relationship for the inhibition of glucose-induced first phase [Ca²⁺]_i increases by HMG-CoA reductase inhibitors in single β-cells. The mean amplitude of the first phase [Ca²⁺]_i increase in response to 8.3 mM glucose was averaged and expressed as the mean±s.e.mean. The number of single β-cells examined was 127 for the control and 18–50 for each experimental condition. The mean amplitude of the first phase [Ca²⁺]_i increase was reduced by simvastatin at 0.1–3 μg ml⁻¹ and simvastatin-acid at 30–100 μg ml⁻¹. }P<0.05 for 0.1 μg ml⁻¹ simvastatin, #P<0.02 for 0.3 μg ml⁻¹ simvastatin and 30 μg ml⁻¹ simvastatin-acid, ##P<0.002 for 100 μg ml⁻¹ simvastatin-acid, ^*P<0.001 for 1 μg ml⁻¹ simvastatin, and ^**P<0.0001 for 3 μg ml⁻¹ simvastatin vs the control.