Figure 5.

Somatostatin-14 and PGE₂-induced cyclic AMP formation (A) and inhibition of somatostatin-14 (1 μM)-stimulated adenylate cyclase activity by the site-specific synthetic peptide G_αs-acetyl-354–372-amide (B) in PTx (100 ng ml⁻¹, 18 h) pre-treated CHOsst₅ membranes. Experiments were performed without forskolin and basal cyclic AMP production was 138±16.8 nmoles mg protein⁻¹ (n=10). Assay conditions were as described in the Methods. Values are means±s.e. mean of 3–4 experiments performed in triplicate.