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Concentration-dependency of somatostatin-14-stimulated [35S]-GTPγS binding to Gαs proteins in CHOsst5 membranes. Membranes were incubated with the indicated concentrations of somatostatin-14 for 2 min and then in the presence of 2 nM [35S]-GTPγS for a further minute at 30°C. Immunoprecipitated 35S-labelled Gαs proteins were then counted. Basal [35S]-GTPγS binding to Gαs was 198±27 c.p.m. Data are the means±s.e.mean of three experiments performed in triplicate.
