Figure 4.

Effect of PKA inhibitors on βAPP maturation in wild type and M146V-presenilin1-expressing HEK293 cells. Stably transfected HEK293 cells overexpressing wt-PS1 or M146V-PS1 were incubated without (control) or in the presence of the indicated concentrations of H89 and PKI then analysed for βAPP (A), APPα (C) and p10 (D) as described in Figure 2. Total Aβ (B) was detected after immunoprecipitation with FCA18, electrophoresis and Western blot with mAbWO2 as described in Methods. Densitometric analyses of total secreted Aβ was quantified as in Figure 2 and are the means ±s.e.mean of three independent experiments.