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Effect of H89 on the secretion of Aβ by TSM1 neocortical neuronal cell line. TSM1 cells were incubated as described in Methods without (control) or in the presence of the indicated concentrations of H89. Total Aβ was detected after immunoprecipitation with FCA18, electrophoresis and Western blot with mAbWO2 as in Figure 4.
