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Inhibition by 12,14-Cl2DHA of GABA-dependent 36Cl− uptake into membrane vesicles prepared from mouse brain. The effect of picrotoxin (PTX) is also shown. Each value represents the mean±s.e.mean of 3–8 independent determinations. Asterisks indicate a statistically significant difference (P<0.05) from controls (Bonferroni's method after one way ANOVA). In these experiments, GABA-dependent uptake was 14.8±1.7 nmol chloride mg protein−1. The structure of 12,14-Cl2DHA is also displayed.
