Figure 6.

(a) A pulse of 1 μM 12,14-Cl₂DHA (horizontal bar) via the Y-tube results in a marked increase in EPSC incidence after circa 10 s exposure. The response was reversed by washing with physiological saline. Cell clamped at −45 mV using gluconate in the recording pipette. (b) Higher resolution images showing similar responses to 10 μM 12,14-Cl₂DHA in a different cell. Top, pretreatment saline blank from the Y-tube; middle, peak response to 12,14-Cl₂DHA showing the marked increase in the incidence of EPSCs; bottom, continued exposure results in a net depression of spontaneous activity (the regular bipolar spikes are residual uncompensated capacative transients and current responses to voltage jumps driven through the patch electrode to gauge membrane input conductance). V_h: −45 mV. (c) At 10 μM in the same cell as (a), 12,14-Cl₂DHA transiently elevated the incidence of EPSCs and IPSCs. Note the sustained effect on the inward currents (downward deflections). The IPSCs appear to be selectively blocked within 2 min.