Figure 3.

Effects of pertussis toxin and cholera toxin on BK-induced IPs accumulation and [Ca²⁺]_i changes in TECs. Cells were preincubated with either pertussis toxin (PTX, 100 ng ml⁻¹) or cholera toxin (CTX, 10 μg ml⁻¹) for 24 h and then exposed to BK (10 μM). (A) [³H]-IPs and (B) [Ca²⁺]_i were determined as described under Methods. Values represent mean±s.e.mean of six separate experiments. The basal level of IPs accumulation was 15,300±2300 d.p.m. well⁻¹.