Figure 8.

Effects of Ca²⁺-channel blockers on BK-induced increase in [Ca²⁺]_i. TECs were preincubated with (A) vehicle, (B) verapamil (10 μM), (C) diltiazem (10 μM), and (D) Ni²⁺ (5 mM) for 30 min and then incubated in Ca²⁺-free buffer. After reaching the steady baseline, the cells were exposed to BK (10 μM), and then Ca²⁺ (1.8 mM) was re-added to the buffer, as indicated by arrows. The data expressed as mean±s.e.mean of five separate experiments are shown in bar graph. ^*P<0.01, as compared with Ca²⁺ influx induced by BK in control cells.