Figure 5.
Co-localization of 3-nitrotyrosine positive proteins and PGI2-synthase in bovine coronary arteries treated with 1 μM or decomposed peroxynitrite. The yellow colourings resulting from a computer-generated overlay of green and red fluorescence indicate areas of the co-localization of anti-PGI2 synthase and anti-nitrotyrosine staining in bovine coronary arteries with or without ONOO− treatment (400 fold magnification). (a) PGI2-synthase stainings in untreated bovine coronary arteries. (b) Computer-generated overlay of red fluorescence (PGI2-synthase staining; a) and green (anti-nitrotyrosine staining; c) resulting from double staining in untreated bovine coronary arteries (overlay of a and c). (c) Anti-nitrotyrosine staining in untreated vessels. (d) PGI2-synthase staining in 1 μM ONOO−-treated vessels. (e) The computer-generated overlay of red (anti-PGI2-synthase staining; d) and green fluorescence (anti-nitrotyrosine staining; f) resulting from double staining in 1 μM ONOO− treated vessels (overlay of d and f). (f) Anti-nitrotyrosine staining in 1 μM ONOO−-treated vessels. (g) Texas red and FITC alone (anti PGI2-synthase and anti-nitrotyrosine omitted). (h) Anti-nitrotyrosine staining in ONOO− treated vessels but in the presence of 10 μM nitrotyrosine.