Figure 1.
Subcutaneous adipose tissue microdialysate glycerol levels (top panel) and ethanol ratio (bottom panel) following L-NMMA treatment. Experimental protocol: 1 h of pre-treatment (probes perfused with Ringer-ethanol), 1 h of solvent or L-NMMA (1 or 10 mmol l−1), 1 h of post-treatment with solvent. L-NMMA versus solvent was statistically tested using ANOVA, followed by Fisher PLSD-test as post-hoc analysis. *Significant differences (P<0.05) from control group (Ringer alone). Microdialysate glycerol levels for both doses of L-NMMA were significantly increased compared to Ringer's solution and also differed from each other. No statistical significant differences were found comparing ethanol ratios. n=9–12 in each group. Mean glycerol levels (μmol l−1) during the pre-treatment period for the different groups were 29±2, 32±3 and 30±3, respectively.