Figure 5.

Inhibition by SR141716A of histamine- and thapsigargin-induced mobilization of cytosolic Ca²⁺. Recordings from fura 2-loaded endothelial cell suspensions that were stimulated with 100 μM histamine (A) or 2 μM thapsigargin (B) in the absence (continuous lines and open circles) and in the presence (dotted lines and closed circles) of 5 μM SR141716A. The cells were first equilibrated for 5 min with or without SR141716A in Ca²⁺-free physiological salt solution. Circles represent mean±s.e.mean of fluorescence ratio values obtained at indicated times. Error bars not visible when the size of the symbol exceeds the spread of s.e.mean. ^*P<0.05 between control and SR141716A-treated groups (n=5–6).