Figure 2.

BK Induces inositol phosphate accumulation in PS cells. (A) PS cell monolayers were labeled with [³H]-inositol for 16 h and then stimulated in serum free medium containing vehicle, BK or [des-Arg¹⁰]KD for 90 min. Incorporation of [³H] into inositol phosphates was determined. Data are represented as the mean fold stimulation over vehicle control±s.e.mean for four independent experiments performed in sextuplicate. ^*P<0.05 compared to vehicle alone. (B) PS cell monolayers were labelled with ³H-inositol for 16 h and then stimulated in serum free medium containing vehicle, BK (10⁻⁸ M), BK (10⁻⁸ M) plus [des-Arg⁹Leu⁸]BK (5×10⁻⁸ M) or BK (10⁻⁸ M) plus Hoe 140 (5×10⁻⁸ M) for 90 min. Incorporation of [³H] into inositol phosphates was determined. Data are represented as the mean fold stimulation over control±s.e.mean for four independent experiments performed in sextuplicate. ^*P<0.001 compared to vehicle alone.