Figure 3.

BK Induces [³H]-thymidine uptake by PS cells. (A) Cultured prostate-derived PS cell monolayers were incubated in RPMI 1640, 0.5% (v v⁻¹) FCS for 72 h. Cells were then exposed to vehicle, BK or [des-Arg¹⁰]KD for 23 h and pulsed for 1 h with [³H]-thymidine. Incorporation of label into DNA was determined. Data are represented as the mean fold stimulation over vehicle control±s.e.mean for six independent experiments performed in sextuplicate. ^*P<0.05 compared to vehicle alone. (B) Cultured prostate-derived PS cell monolayers were incubated in RPMI 1640, 0.5% (v v⁻¹) FCS for 72 h. Cells were then exposed to vehicle, BK (10⁻⁸ M), BK (10⁻⁸ M) plus [des-Arg⁹Leu⁸]BK (5×10⁻⁸ M) or BK (10⁻⁸ M) plus Hoe 140 (5×10⁻⁸ M) for 23 h and pulsed for 1 h with [³H]-thymidine. Incorporation of label into DNA was determined. Data are the mean±s.e.mean for four independent experiments performed in sextuplicate. ^*P<0.001 compared to vehicle alone.