Figure 1.

Effect of the NO-donors GSNO (A) and spermine-NO (B) on IL-1β stimulated sPLA₂-IIA activity in mesangial cells. Cells were incubated in serum-free DMEM with 0.1 mg ml⁻¹ fatty acid-free BSA for 24 h. Then they were treated for 24 h with IL-1β (2 nM) or vehicle in the absence or presence of different concentrations of the NO-donors as indicated. sPLA₂-IIA activity was measured in the cell culture supernatant as described in Methods. Each value represents the means±s.e.mean of three parallel determinations per experiment. The experiment was repeated four times with similar results. Statistical analysis was performed by ANOVA. ^*P<0.05; n.s., not significant