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. 1999 May;127(1):65–74. doi: 10.1038/sj.bjp.0702506

Figure 8.

Figure 8

Effects of PDE inhibitors and isoprenaline on [cAMP]i in isolated rat ventricular myocytes. The selective PDE inhibitors MIMX (1 μM), EHNA (10 μM), cilostamide (0.1 μM) and Ro20-1724 (0.1 μM) were tested as indicated in three different preparations of rat ventricular myocytes (corresponding to the different symbols used in A and B) in the absence (A) or presence (B) of 1 nM isoprenaline. The effects are expressed in % increase over basal cyclic AMP concentration which was, respectively, 59 (○), 52 (▪) and 22 (•) pmoles per mg protein, assuming that each myocyte contained 15 ng protein. Each individual measurement was done in triplicate. The bars indicate the average response in each condition. Separate experiments were performed with IBMX (100 μM, A) and isoprenaline (1 μM, B), and the corresponding bars indicate the mean and the s.e.mean of the number of preparations indicated near the bars. Statistical significant differences from basal cyclic AMP concentration are indicated as: *P<0.05.