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Reversibility of the inhibitory effect of neutral sphingomyelinase (upper panel) and C8-ceramide (lower panel) on LNCaP cell proliferation. Cells were treated at day 0 and day 2 with vehicle, neutral sphingomyelinase (N SMase, 200 mU ml−1) and C8-ceramide (C8-cer, 10 μM) or with N SMase or C8-cer at day 0 and vehicle at day 2. Data (mean±s.e.mean) are from one experiment representative of 3–4.
