Figure 3.

Ionomycin reduced cyclic AMP-mediate inhibition of IL-5 production. Cells (10⁵ ml⁻¹) were stimulated with immobilized OKT3 (10 μg ml⁻¹) in the presence of 1 μM PGE₂, 10 μM forskolin or 100 μM db-cAMP. Ionomycin at designated concentrations was included from the start of some cultures. Culture supernatants were harvested after 24 h and assayed for IL-5 by EIA. The data are the mean±s.e.mean of the per cent inhibition of TCR-induced IL-5 production from four separate experiments.