Figure 7.

TCR-stimulated mobilization of [Ca²⁺]_i in human T cells. Fura-2-loaded cells (5×10⁶ ml⁻¹) were stimulated by crosslinking pretreated OKT3 using anti-mouse IgG antibody. OKT3 (2 μg ml⁻¹) and crosslinking secondary antibody (20 μg ml⁻¹) were added at −2 and 0 min, respectively. The mean±s.e.mean of [Ca²⁺]_i during the time course are shown (n=4 separate experiments). Some s.e.mean bars are within the symbol. The peak and sustained phase increase in [Ca²⁺]_i were assessed at 1 and 5 min after the addition of secondary antibody respectively in the following experiments.