Figure 3.

The effect of actinomycin D on endothelium-dependent relaxations to (a) des-arg⁹-bradykinin (3 μM) and (b) bradykinin (3 μM) in U46619-contracted pig coronary artery ring preparations. Tissues were either untreated (control), treated with actinomycin D (2 μM) or treated with actinomycin D (2 μM) and thermolysin (0.01–1 u ml⁻¹, 20 min). Responses were examined after 3 or 13 h of incubation at 37°C. Data are mean±s.e.mean from four experiments and are expressed as percentages of the maximum relaxation to isoprenaline (1 μM). ^*P<0.05 (t-test) vs 3 h response. †P<0.05 (t-test) vs untreated control.