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Effect on gene capture of length of nonhomologous sequences placed at the end of the hook. The diagram shows only one of the targeting hooks of the pVC-SV60-polylinker TAR vector. The vector was cut with a set of endonucleases to release the SV40-specific targeting hook with a different length polylinker (A). Yield of transgene-positive clones obtained with different TAR constructs (B).
