Table 3.
Isolation of the mouse transgene Tg.AC and the human HPRT gene with ARS-containing TAR vectors
| Targeted region | Experiment | Mean No. of transformants per ug vector (range) | No. of gene-positive clones/No. of clones analyzed (%) |
| Tg.AC mouse transgene | 1 | 86 (60–120) | 3/250 (1.2) |
| 2 | 319 (242–380) | 2/400 (0.5) | |
| 3 | 99 (52–166) | 3/300 (1.0) | |
| Control* | 1,910 (1,132–8,600) | 0/3000 (0.0) | |
| Human HPRT | 1 | 72 (52–108) | 1/300 (0.33) |
| 2 | 24 (10–60) | 1/150 (0.67) | |
| 3 | 22 (8–38) | 1/175 (0.57) |
*Control TAR cloning experiment was carried out with the same TAR vector but transformants were selected on SD medium lacking 5-FOA