Figure 10.
Expression of bcl-2, bax, c-myc, fas and fasL in ET-18-OCH3-treated mitogen-activated T-lymphocytes. Human T-cells activated with PHA and IL-2 as described in the Methods section were incubated with 10 μM ET-18-OCH3 for the indicated times, and then total RNA was purified and subjected to semiquantitative RT–PCR analysis with oligonucleotide primers specific to bcl-2, bax, c-myc, fas and fasL. PCR amplification of β-actin was used as an internal control. After 18 cycles (β-actin), 22 cycles (bax and fas), 25 cycles (bcl-2 and c-myc) and 35 cycles (fasL), shown to be at the linear phase of amplification, the PCR products were electrophoresed onto a 2% agarose gel and stained with ethidium bromide.