Figure 5.

Incorporation of ET-18-OCH₃ in human resting and mitogen-activated T-lymphocytes. Human resting T-lymphocytes (solid circles) and T-cells activated for 4 days with 0.5 μg ml PHA and for 1 additional day with 0.5 μg ml⁻¹ PHA and 50 U ml⁻¹ IL-2 (solid squares) were incubated with 5 μg ml⁻¹ ET-18-OCH₃ and 0.05 μCi ml⁻¹ of [³H]-ET-18-OCH₃ for the times indicated to measure incorporation of the ether lipid into the cell as described in the Methods section. Data are shown as mean values±s.e.mean. from three independent experiments.