Figure 3.

Micromass cultures of Hoxc-8 transgenic cells. Primary rib chondrocytes were prepared from newborn mice and cultured in micromass as described. Histological staining with (Hematoxylin & Eosin Panels A, E), and Alcian Blue, (Panels B, F) and immunohistochemical detection of Collagen II (Panels C, G, I) and Collagen X (Panels D, H, J) on sections prepared on day 2 (Panels A-D), day 5 (Panels E-H), and day 7 (Panels I, J) do not indicate differences to control chondrocyte cultures (see Figure 1) even at early timepoints after plating.