Figure 6.

RT–PCR results. Two μg of total RNA from human umbilical artery was reverse transcribed using oligo-(dT) either with (+) or without (−) reverse transcriptase, and the cDNAs were amplified by PCR using pairs of primers C1 C1′ (5-HT_2A); C2 C2′ (5-HT_1B) and C3 C3′ (5-HT_1D). Human brain cDNA was amplified with the primer pair C3, C3′. Products were separated by polyacrylamide (5%) gel electrophoresis and visualized by ethidium bromide staining. HaeIII-digested ΦX-174 RF DNA molecular size markers of indicated size (Pharmacia) were run in lanes on the left of each gel.