Figure 2.

UTP-dependent UDP-glucose pyrophosphorylase-catalyzed conversion of [¹⁴C]-glucose-1P to [¹⁴C]-UDP-glucose. HPLC tracings corresponding to 1 h incubations in the presence of 0.5 units ml⁻¹ of both UDP-glucose pyrophosphorylase and inorganic pyrophosphatase, 1 μM [¹⁴C]-glucose-1P and either vehicle (a), 100 nM UTP (b) or 100 μM ATP (c). The data are representative of at least three experiments performed with duplicate samples. The elution time of glucose-1P (Gluc-1P), UDP-glucose (UDPG), and ADP-glucose (ADPG) are indicated with arrows on top of a.