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Reverse-phase HPLC separation of eotaxin from supernatants of stimulated airway smooth muscle cells. The supernatant was separated by C18 HPLC and 0.5 min fractions were collected. Immunoreactive eotaxin was detected with a major peak at 34.5–35 min and a minor peak at 36–36.5 min. The position for RANTES is shown for comparison.
