Figure 4.

Effect of pretreatment with SP (a) and NKA (b) antisera on nociceptin-induced scratching, biting and licking response in mice. The antisera against SP and NKA was preinjected i.t. 5 min prior to i.t. injection of nociceptin (3.0 fmol). First and second injections were done in a 5 μl volume, separately. The duration of scratching, biting and licking induced by nociceptin was determined over a 20 min period starting immediately after the second injection. Each value represents the mean±s.e.mean of ten mice in each group. ^*P<0.05 when compared with CSF-controls ((a) 97.6±13.8 s; (b) 101.5±4.9 s).