Figure 6.

Fluocinolone suppressed apoptosis triggered by co-treatment with TNF-α and cycloheximide. Bovine glomerular endothelial cells were exposed to increasing concentrations of cycloheximide (control), 1 ng ml⁻¹ TNF-α plus cycloheximide, and 1 ng ml⁻¹ TNF-α plus cycloheximide plus 10 nM fluocinolone (A); or 5 ng ml⁻¹ LPS plus cycloheximide, and 5 ng ml⁻¹ LPS plus cycloheximide plus 10 nM fluocinolone (B). The amount of DNA fragmentation was determined with the diphenylamine reaction. Values are means±s.e.mean of four individual experiments.