Figure 3.

Influence of glibenclamide pretreatment of mouse islets on the kinetics of glucose-induced insulin secretion. The islets were cultured for 18–20 h in a control medium (Ctrl) or in a medium containing 0.01 μM glibenclamide (Glib). After preincubation without or with glibenclamide, batches of 25 islets were then perifused with a medium that did not contain glibenclamide. The glucose concentration was increased from 3 to 15 mM as indicated. Results are presented as absolute rates of secretion (A) or as a percentage of the islet insulin content (B), which is shown in the inset. Values are means±s.e.mean for five separate experiments.