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Absence of 35 kDa pro-IL-1β in LPS primed 5 mM ATP-treated THP-1 cell supernatants. Samples were prepared as described in the Methods section. Proteins were resolved on 15% polyacrylamide gels and electrophoretically transferred onto nitrocellulose. Lane 1: THP cell lysates; Lane 2: Cell supernatants. The immunoreactivity detected had the electrophoretic mobility expected for recombinant human IL-1β (lane 3).
