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Effect of antagonists on LPS-induced IL-1β release in (A) THP-1 cells and (B) freshly isolated adherent monocytes. KN-62, PPADS and oxidized ATP at the concentrations indicated were preincubated for 30 min (2 h for oxidized ATP) with the cells prior to LPS stimulation. Data are the mean (with 95% confidence limits) of at least three separate determinations.
