Fig. 3.

Accumulation of A5 HCs and LCs in N. benthamiana leaves coinfected with TMV and PVX provectors. (A) The LC is expressed with TMV and the HC with PVX. (B) The LC is expressed with PVX and the HC with TMV. Proteins are separated in 12% polyacrylamide gels run under reducing conditions. (B Top) Coomassie blue staining. (B Middle) Western blot probed with HRP-conjugated goat anti-human IgG (γ-chain-specific) antibodies (Sigma). (B Bottom) Western blot probed with HRP-conjugated rabbit anti-human IgG (λ-chain-specific) antibodies (Sigma). U, uninfected tissue; 3–11, dpi; S, A5 standard; L, LC expressed alone with TMV provectors (6 dpi); H, HC expressed alone with TMV provectors (6 dpi); RbcL, large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase.