Fig. 5.

Accumulation of mAb A5 in N. benthamiana leaves after coinfiltration with 35S-promoter constructs or viral vectors, analyzed by using antigen-specific ELISA. Serial 2-fold dilutions of crude extracts from leaves coinfected with viral provectors (PVX-HC + TMV-LC, 8 dpi; TMV-HC + PVX-LC, 10 dpi) and 35S-promoter constructs (35S-LC + 35S-HC, 2 dpi) were compared with serial dilutions of the mAb A5 standard expressed in CHO cells and crude protein extracts from uninfected tissue (uninf) used as a negative control.