Fig. 6.

Purification of the A5 mAb by using protein A magnetic beads. (A) Plant-derived and standard mAbs migrating in a 10% polyacrylamide gel under nonreducing conditions. (B) Plant-derived and standard mAbs resolved in a 12% polyacrylamide gel under reducing conditions. (B Top) PAGE with Coomassie blue staining. (B Middle) Western blot probed with γ-chain-specific antibodies. (B Bottom) Western blot probed with λ-chain-specific antibodies. Lane 1, PVX-HC + TMV-LC, crude extract; lane 2, TMV-HC + PVX-LC, crude extract; lane 3, PVX-HC + TMV-LC, purified mAb; lane 4, TMV-HC + PVX-LC, purified mAb; lanes 5 and 6, A5 standard.