TABLE 3.
Comparison of Pine and Montane Voles: CRFR1 Binding Using 125-I-Sauvagine and CRFR2 Subtraction
| Region | Abbreviation | Meadow Female Mean ± SEM | Meadow Male Mean ± SEM | Prairie Female Mean ± SEM | Prairie Male Mean ± SEM |
|---|---|---|---|---|---|
| Cortical | |||||
| Prefrontal | PFCtx** | 1944 ± 345 | 1951 ± 124 | 777 ± 364 | 974 ± 184 |
| Insular | IFCtx** | 2839 ± 572 | 2904 ± 252 | 1246 ± 362 | 1165 ± 77 |
| Orbitofrontal | OFCtx* | 2873 ± 324 | 2813 ± 218 | 1261 ± 519 | 1757 ± 372 |
| Cingulate rostral | CgCtx1** | 1943 ± 119 | 1838 ± 225 | 980 ± 217 | 1145 ± 162 |
| Cingulate middle | CgCtx2** | 1553 ± 122 | 1700 ± 132 | 837 ± 307 | 959 ± 53 |
| Cingulate caudal | CgCtx3 | 1395 ± 59 | 1733 ± 227 | 711 ± 297 | 1137 ± 303 |
| Retrosplanial | RSCtx** | 1681 ± 339 | 1213 ± 159 | ND | 499 ± 151 |
| Septal-hippocampal | |||||
| Lateral septum | LS | ||||
| dorsal rostral | rLSD | 3445 ± 895 | 4009 ± 861 | 3149 ± 2178 | 1949 ± 764 |
| intermediata rostral | rLSI | 3845 ± 1191 | 5609 ± 1001 | 5427 ± 4015 | 4297 ± 1540 |
| dorsal caudal | cLSD | 4821 ± 960 | 4470 ± 456 | 8864 ± 5168 | 7790 ± 2827 |
| intermediata caudal | cLSI | 6817 ± 1208 | 6657 ± 338 | 4054 ± 2508 | 8395 ± 3563 |
| Hippocampus | HC | ||||
| CA1 | CA1 | 651 ± 65 | 527 ± 44 | ND | 573 ± 50 |
| CA2 | CA2 | 549 ± 120 | 533 ± 69 | ND | 465 ± 45 |
| CA3 | CA3 | 657 ± 104 | 766 ± 102 | ND | 538 ± 85 |
| dentate gyrus | DG | 547 ± 144 | 775 ± 27 | ND | ND |
| Extended amygdala | |||||
| Bed nucleus of the stria terminals | BnST | ||||
| medial | mBnST | 835 ± 185 | 907 ± 331 | 836 ± 326 | 1247 ± 601 |
| lateral | lBnST | 1582 ± 378 | ND | ND | 453 ± 199 |
| caudal | cBnST | 3863 ± 2189 | 2206 ± 556 | 3082 ± 1761 | 4059 ± 507 |
| Amygdala | Amyg | ||||
| central | CeA | 691 ± 135 | 740 ± 160 | 672 ± 179 | 980 ± 497 |
| basolateral rostral | rBLA | 589 ± 456 | 1340 ± 679 | 492 ± 86 | 813 ± 475 |
| medial rostral | rMeA | 1848 ± 738 | 1281 ± 318 | 952 ± 679 | ND |
| cortical rostral | rCoA | 1944 ± 279 | 2071 ± 380 | 970 ± 258 | 947 ± 178 |
| basolateral caudal | cBLA | 1221 ± 171 | 1430 ± 175 | ND | 498 ± 169 |
| medial caudal | cMeA | 2115 ± 544 | 1375 ± 43 | 858 ± 537 | 681 ± 208 |
| cortical caudal | cCoA | 1834 ± 169 | 2425 ± 296 | 799 ± 195 | 1148 ± 260 |
| Striatum and habenula | |||||
| Nucleus accumbens | NAcc | ||||
| shell | shNAcc** | 4335 ± 608 | 4598 ± 719 | 977 ± 106 | 1043 ± 83 |
| septal pole | spNAcc | 1512 ± 348 | 1956 ± 198 | 2344 ± 944 | 3307 ± 729 |
| Caudate-putamen | CPu | ||||
| dorsal | CPuD* | 2032 ± 468 | 2000 ± 435 | 967 ± 233 | 955 ± 181 |
| ventral | CPuV | 1353 ± 247 | 1612 ± 195 | 1137 ± 293 | 1247 ± 259 |
| Habenula | |||||
| medial | mHab | 781 ± 84 | 658 ± 75 | ND | 540 ± 163 |
| lateral | lHab | 730 ± 177 | 584 ± 124 | 883 ± 388 | 458 ± 170 |
| Thalamus | |||||
| laterodorsal | ldThal** | 3380 ± 552 | 3384 ± 802 | ND | 563 ± 127 |
| Olfactory and hindbrain | |||||
| Olfactory bulb | |||||
| external plexiform | OB* | 11849 ± 1897 | 9461 ± 3408 | 5080 ± 1477 | 5787 ± 2041 |
| Superior colliculus | SColl** | 8202 ± 1908 | 7970 ± 945 | 1522 ± 279 | 1982 ± 319 |
| Periaqueductal gray | PAG | 752 ± 47 | 888 ± 50 | 599 ± 174 | ND |
| Dorsal raphe | DR | 3891 ± 1250 | 2991 ± 1109 | 1728 ± 1311 | 10495 ± 2245 |
| Locus coeruleus | LC* | 1365 ± 205 | 1226 ± 112 | 540 ± 389 | 484 ± 130 |
| Pontine dorsal tegmental n. | PDTg* | 796 ± 140 | 971 ± 170 | ND | 452 ± 188 |
| Principal motor nucleus of 5 | Pr5 | 1834 ± 276 | 1951 ± 630 | 2826 ± 879 | 1969 ± 742 |
| Cerebellum | |||||
| granular layer | CBg | 2222 ± 424 | 3399 ± 279 | 2396 ± 323 | 1966 ± 565 |
Values represent mean DPM/mg tissue from n = 4 per group.
ND, not detected (<2 S.D. nonspecific binding);
*
P < 0.05 species effect;
**
P < 0.01 species effect;
†
P < 0.05 sex effect.