TABLE 4.
Comparison of Pine and Montane Voles: CRFR2 Binding Using 125-I-Sauvagine and CP-154,5261
| Region | Abbreviation | Meadow Female Mean ± SEM | Meadow Male Mean ± SEM | Prairie Female Mean ± SEM | Prairie Male Mean ± SEM |
|---|---|---|---|---|---|
| Cortical | |||||
| Prefrontal | PFCtx | 1390 ± 186 | 1033 ± 130 | 1913 ± 200 | 1567 ± 84 |
| Insular | IFCtx** | 1181 ± 99 | 987 ± 74 | 1667 ± 123 | 1490 ± 109 |
| Orbitofrontal | OFCtx | 1475 ± 130 | 1187 ± 127 | 2774 ± 530 | 1842 ± 143 |
| Cingulate rostral | CgCtx1* | 1351 ± 136 | 1151 ± 55 | 1751 ± 154 | 1699 ± 123 |
| Cingulate middle | CgCtx2** | 1286 ± 142 | 1118 ± 58 | 1772 ± 202 | 1820 ± 54 |
| Cingulate caudal | CgCtx3** | 1277 ± 150 | 1137 ± 91 | 1871 ± 179 | 2023 ± 169 |
| Retrosplenial | RSCtx | 1608 ± 200 | 1266 ± 57 | 1558 ± 150 | 1290 ± 94 |
| Septal-hippocampal | |||||
| Lateral septum | LS | ||||
| dorsal rostral | RLSD** | 9891 ± 2333 | 4869 ± 306 | 27167 ± 2369 | 30216 ± 4144 |
| intermediata rostral | RLSI** | 10054 ± 1655 | 7136 ± 654 | 23646 ± 2990 | 29935 ± 2470 |
| dorsal caudal | CLSD** | 8139 ± 1158 | 7593 ± 241 | 34474 ± 7817 | 32930 ± 3591 |
| intermediata caudal | CLSI* | 13629 ± 2659 | 8774 ± 453 | 24334 ± 5494 | 31306 ± 5716 |
| Hippocampus | HC | ||||
| CA1 | CA1* | 1216 ± 119 | 1123 ± 127 | 2422 ± 398 | 1750 ± 139 |
| CA2 | CA2 | 1360 ± 51 | 1088 ± 106 | 1784 ± 389 | 1492 ± 102 |
| CA3 | CA3** | 1110 ± 49 | 1022 ± 104 | 1596 ± 117 | 1613 ± 153 |
| dentate gyrus | DG | 1401 ± 50 | 1130 ± 27 | 1497 ± 133 | 1481 ± 95 |
| Extended amygdala | |||||
| Bed nucleus of the stria terminals | BnST | ||||
| medial | mBnST | 1945 ± 236 | 1603 ± 166 | 2508 ± 384 | 2675 ± 343 |
| lateral | lBnST | 1552 ± 426 | 1916 ± 247 | 2422 ± 313 | 1775 ± 239 |
| caudal | cBnST† | 2954 ± 1195 | 7088 ± 2378 | 5375 ± 1914 | 8536 ± 609 |
| Amygdala | Amyg | ||||
| central | CeA | 1043 ± 52 | 1017 ± 133 | 1169 ± 116 | 1143 ± 113 |
| basolateral rostral | rBLA | 2316 ± 393 | 1675 ± 452 | 1292 ± 172 | 1199 ± 148 |
| medial rostral | rMeA | 1442 ± 324 | 1438 ± 347 | 2264 ± 429 | 2927 ± 917 |
| cortical rostral | rCoA | 1470 ± 79 | 1242 ± 124 | 1504 ± 235 | 1702 ± 224 |
| basolateral caudal | cBLA | 1230 ± 101 | 1323 ± 107 | 1339 ± 181 | 1300 ± 93 |
| medial caudal | cMeA | 1168 ± 84 | 1318 ± 90 | 2237 ± 219 | 1485 ± 123 |
| cortical caudal | cCoA | 1593 ± 177 | 1393 ± 372 | 1455 ± 172 | 2222 ± 707 |
| Striatum and habenula | |||||
| Nucleus accumbens | NAcc | ||||
| shell | shNAcc | 1068 ± 54 | 1240 ± 321 | 1560 ± 166 | 1474 ± 160 |
| septal pole | spNAcc** | 1496 ± 193 | 949 ± 23 | 10608 ± 2223 | 13684 ± 4451 |
| Caudate-putamen | CPu | ||||
| dorsal | CPuD** | 820 ± 35 | 791 ± 45 | 1095 ± 122 | 982 ± 32 |
| ventral | CPuV* | 1071 ± 60 | 866 ± 55 | 1630 ± 177 | 1310 ± 158 |
| Habenula | |||||
| medial | mHab | 935 ± 40 | 814 ± 35 | 1129 ± 134 | 1037 ± 118 |
| lateral | lHab* | 1042 ± 53 | 965 ± 57 | 1325 ± 91 | 1167 ± 72 |
| Thalamus | |||||
| laterodorsal | ldThal** | 5308 ± 1117 | 7582 ± 1027 | 504 ± 39 | 1224 ± 125 |
| Olfactory and hindbrain | |||||
| Olfactory bulb | |||||
| external plexiform | OB* | 1587 ± 48 | 1611 ± 48 | 3023 ± 224 | 3778 ± 944 |
| Superior colliculus | SColl | 862 ± 146 | 1227 ± 157 | 1323 ± 267 | 1218 ± 35 |
| Periaqueductal gray | PAG | 984 ± 93 | 821 ± 68 | 1529 ± 178 | 1197 ± 129 |
| Dorsal raphe | DR** | 10575 ± 547 | 10593 ± 1377 | 19083 ± 2604 | 16370 ± 1686 |
| Locus coeruleus | LC | 827 ± 96 | 895 ± 41 | 1363 ± 190 | 1041 ± 86 |
| Pontine dorsal tegmental n. | PDTg | 863 ± 51 | 758 ± 90 | 1417 ± 159 | 1035 ± 92 |
| Principal motor nucleus of 5 | Pr5 | 4317 ± 706 | 3721 ± 531 | 4692 ± 606 | 5250 ± 291 |
| Cerebellum | |||||
| granular layer | CBg** | 932 ± 77 | 742 ± 97 | 1315 ± 145 | 1301 ± 102 |
1
Values represent mean DPM/mg tissue from n = 4 per group.
ND, not detected (<2 S.D. nonspecific binding);
*
P < 0.05 species effect;
**
P < 0.01 species effect;
†
P < 0.05 sex effect.